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Canadian Journal of Gastroenterology and Hepatology
Volume 2019, Article ID 5672795, 11 pages
Review Article

Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis

1Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
2Federal Institute of Education, Science and Technology of Ceará, Campus Fortaleza, Ceará, Brazil
3Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil

Correspondence should be addressed to Livia Melo Villar; moc.liamg@zurcoifaivil

Received 13 October 2018; Accepted 3 February 2019; Published 1 April 2019

Academic Editor: Kevork M. Peltekian

Copyright © 2019 Livia Melo Villar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or high-risk groups. The main objective of this review is to give an insight about the usefulness of oral fluid and DBS for detecting HBV markers. Several groups have evaluated the detection of HBsAg, anti-HBc, and anti-HBs markers in oral fluid and DBS samples demonstrating 13 to 100% of sensitivity and specificity according different groups, sample collectors, and diagnosis assays. In the same way, HBV DNA detection using oral fluid and DBS samples demonstrate different values of sensitivity according type of collection, studied group, extraction, and detection methods. Thus, serological and molecular diagnostic tests demonstrated good performance for detecting HBV using oral fluid and DBS according some characteristics and could be useful to increase the access to the diagnosis of HBV.