|
| Reference | Population | Country | DNA Extraction Method (DBS) | Amplification Method | Sensitivity (%) | Specificity (%) |
|
| [38] | HBsAg positive serum + negative whole blood | India | Not performed | Simple PCR (core gene) | NR | NR |
| [39] | HBsAg positive patients (82) | Spain | QIAamp mini columns | Real Time PCR (core gene) | 88 | 100 |
| [40] | HBsAg positive patients (47) | Mexico | QIAamp® DNA micro kit | Cobas Amplicorv1.5 | 100 | NR |
| [41] | 60 patients with undetermined serological Status | China | Chelex-100 | Nested PCR (pre-core/core gene) | NR | NR |
| [42] | HBsAg positive patients (50) | Egypt | Not performed | Direct amplification (KAPA blood PCR Kit) | 100 | 100 |
| HBsAg negative patients (10) |
| [31] | HBV DNA positive patients (100) | Germany | MagNaPure 96 system using Viral NA Universal kit | Artus HBV LC PCR
VERSANT HBV bDNA 3.0 assay | 93 | 100 |
| HBV DNA negative patients (50) |
| [32] | HBV DNA positive patients (50) | France | FTA purification reagent | Cobas Taqman HBV test V2.0 | 98 | NR |
| HBV DNA negative patients (10) |
| [43] | HBsAg positive patients (26) | Congo | COBAS®
AmpliPrep/COBAS® Taqman® HBV test v1.0 | COBAS® AmpliPrep/COBAS® Taqman® HBV test v1.0 | 96 | NR |
| [29] | HBV DNA positive patients (100) | Germany | Not informed | Not informed | 93 | NR |
| [44] | HBV DNA positive and HIV-infected patients (68) | Zambia | Pre-extraction buffer | AmpliPrep/COBAS TaqMan HBV test | 91 | NR |
| [45] | HBV viral load value range from 2.14 log to >7 log IU/ml | Ethiopia | Abbott RealTime HBV assay on sp2000 extractor | rt2000realtime PCR instrument | 88 | NR |
| [46] | Hepatitis B-infected patients (85) | Denmark | Not performed | Ultrio Elite assay | 97.6 | NR |
| Blood donors negative for HBV infection (99) |
|
