Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats

Table 1

Primer sequences used in this study.

Primer	Sequence	Size (bp)
AMPK-1α	F: 5′-CAA-AGA-TTT-CTA-CTT-GGC-AAC-AAG-C-3′; R: 5′-CAA-CCA-AGA-ATG-GTA-CTC-TCT-CAG-G-3′	95
AMPK-2α	F: 5′-CGC-ACT-GTG-AAA-TCA-CAT-ACA-CTC-T-3′; R: 5′-GTC-ACC-TGG-GAA-TGT-TCA-CAT-AAC-T-3′	95
CPT1	F: 5′-GCT-TAT-CTT-CTA-ATC-CCA-CCC-AGT-C-3′; R: 5′-CCA-TTA-CTT-GAG-ATC-CCT-GAG-TCT-G-3′	99
PGC-1α	F: 5′-CCT-GAA-CTT-GAC-CTT-TCT-GAA-CTT-G-3′; R: 5′-ATT-GGT-CAC-TAC-ACC-ACT-TCA-ATC-C-3′	85
GAPDH	F: 5′-CCT-TCT-CTT-GTG-ACA-AAG-TGG-ACA-T-3′; R: 5′-CGT-GGG-TAG-AGT-CAT-ACT-GGA-ACA-T-3′	96

AMPK-1α, AMP-activated protein kinase-1α; AMPK-2α, AMP-activated protein kinase-2α; CPT1, carnitine palmitoyltransferase 1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1α; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; and R, reverse.