Research Article
Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis
Figure 7
Comparison of SMMC7721 cell line tumorigenicity and induction of angiogenesis with caveolin-1 siRNA or negative control in vivo. (a) The final tumors were photographed immediately after dissection. Their relative sizes in the spleen (1, 2) are shown against a ruler. The maximal tumor volume in the caveolin-1 siRNA-LV (KD) and negative control (NC) groups was (347.2 ± 101.7) and (735.6 ± 159.1) mm3, respectively . The numbers of metastatic tumors in the liver (3, 4) for the caveolin-1 siRNA-LV (KD) and negative control (NC) groups were (0.9 ± 0.3) and (3.5 ± 1.9), respectively . (b) Immunohistochemical staining of VEGF in tumor sections viewed at 400x magnification; VEGF is stained dark brown. Compared to the NC group, the VEGF levels in the KD group were significantly lower . (c) Immunohistochemical staining of CD34 in tumor sections viewed at 400x magnification; CD34 is stained dark brown. Compared to the NC group, the MVD of the KD group was significantly lower .
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