Liver Resection Promotes (Regulates) Proinflammatory Cytokines in Patients with Hepatocellular Carcinoma

Table 1

Primer sequences and other information used to determine the expressions of pro- and anti-inflammatory cytokines.

Gene	Primer sequence	Amplicon size (bp)	Annealing temperatures (°C)
IL-1α	Forward (5′-3′); GTGACTGCCCAAGATGAAGACC; Reverse (5′-3′); TCCCAGAAGAAGAGGAGGTTGG	101	61
IL-6	Forward (5′-3′); AGAGTAACATGTGTGAAAGCAGCA; Reverse (5′-3′); TGATGATTTTCACCAGGCAAGTCT	129	61
IL-1β	Forward (5′-3′); AGGGACAGGATATGGAGCAACA; Reverse (5′-3′); CTTTCAACACGCAGGACAGGT	129	61
IL-10	Forward (5′-3′); TCAAGGCGCATGTGAACTCC; Reverse (5′-3′); CATTCTTCACCTGCTCCACGG	120	61
TGF-β1	Forward (5′-3′); CATGCCAACTTCTGCCTCGG; Reverse (5′-3′); TGGTTGTACAGGGCCAGGAC	83	61
TNF-α	Forward (5′-3′); GACCTCTCTCTAATCAGCCCTCT; Reverse (5′-3′); CTGGTTATCTCTCAGCTCCACG	174	61