A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma

Figure 1

Viability, cytotoxicity, and caspase-activity assays in HepG2 and DLD-1 cells. (a) A very pronounced limitation of HepG2 viability in terms of a cytostatic effect already was observed after a 2-hour incubation with I-ol, as indicated by the relative fluorescence units (RFUs) observed. (b) Comparable effects on the viability of DLD-1 cells after 8 h with I-ol. (c) Reduced viabilities of HepG2 cells treated with smaller I-ol concentrations after 8 h only at the highest concentration. (d) No cytostatic effect on HepG2 cell viability was caused by treatment with cyclodextrin. (e) A strong increase in relative caspase activities of HepG2 cells treated with the highest I-ol concentration was found after an 8-hour incubation. (f) Maximum relative cytotoxic effects observed in HepG2 cells treated with the highest I-ol concentration after 24-hour incubation. The significance values from the “simple main effect of time” are shown below the group designations. The results are expressed as the mean ± SD. Statistical calculations were performed based on two-way mixed ANOVA, followed by Bonferroni’s post hoc test. In all cases, the significance level was (significant), (highly significant), and (very highly significant). Dose-dependent effects of I-ol were calculated by determining Pearson’s correlation coefficient (r) and performing linear-regression analysis (adjusted R²) with the following statistical parameters: (significant), (highly significant), and (very highly significant).

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