TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell

Figure 4

TRIM29 physically binds with mutant P53. (a) qRT-PCR was used to detect the expression of TRIM29 and P53 in the three drug-resistant cell models with the increase of drug resistance concentration. After the HT29-OX-12 μmol/L cells were transfected with pIRES2-ZsGreen1-TRIM29-flag plasmid, the changes of P53 and MDR1 expression were detected. (b) The Western blot method was used to detect the expression of TRIM29 and P53 in the three drug-resistant cell models. (c) The quantitative analysis of (b). (d) After the HT29-OX-12 μmol/L cells were transfected with pIRES2-ZsGreen1-TRIM29-flag plasmid, the protein expression changes of P53 and MDR1 expression were detected. (e) Quantitative analysis of (d). (f) Coimmunoprecipitation method was used to detect the interaction between TRIM29 and mutant P53 protein in HT29 cells. (g) Immunofluorescence detection of the interaction between TRIM29 and mutant P53 protein in HT29 cells. Scale bar = 100 μm. The magnification is 400 times; ns: not significant; ; . The unpaired t-test was used to analyze comparisons between two groups, and comparisons among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test.

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