Research Article
ptk2 and mt2a Genes Expression in Gastritis and Gastric Cancer Patients with Helicobacter pylori Infection
Table 1
Primers of H. pylori T4SS, 16s rDNA, and vacA in simple PCR.
| | Specific primers | Sequence | Annealing Tm°C | Product size bp | Reference |
| 16s rDNA H. pylori F
16s rDNA H. pylori R | CTGGAGAGACTAAGCCCTCC
AGGATCAAGGTTTAAGGATT | 50 | 446 | This study |
| cagA F
cagA R | TGACCAACAACCACAAACCG
TCAGGATCGTATGAAGCGACAG | 57 | 108 | This study |
| cagA EPIYA-C F
cagA EPIYA-C R | AAGAAAGCAGGACAAGCAGC
CTAACCGATCGCCCTACCTT | 55 | 188 | This study |
| cagT F
cagT R | AGGGTGTGGTGATGATAGCG
TGCTTGTTGTTTGCTCCACT | 55 | 154 | This study |
| cagE F
cagE R | GAATGGAGCGAGCGATGAAA
TAGGAATTTGCAGCGCTCAC | 56 | 163 | This study |
| cagY F
cagY R | AGTTCAAGTGGCGCTAGATTG
ACAAGCCTTTCAAGCATTCGT | 57 | 200 | This study |
| vacA s1/s2 F
vacA s1/s2 R | ATGGAAATACAACAAACACAC
CTGCTTGAATGCGCCAAAC | 55 | 259/286 | Atherton et al. [46] |
| vacA m1/m2 F
vacA m1/m2 R | TGGATAGTGCGACTGGGTTT
TCCATGCGGTTGTTGTTGTT | 54 | 205/220 | This study |
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The primers yield various fragments of vacA depending on the presence of a repetitive nucleotides sequence in some vacA alleles. |
