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Canadian Journal of Infectious Diseases
Volume 1 (1990), Issue 2, Pages 51-56
Original Article

Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae

S Wong and LE Bryan

Departments of Microbiology and Infectious Diseases, University of Calgary and Foothills Hospital, Calgary, Alberta, Canada

Received 30 January 1990; Accepted 13 May 1990

Copyright © 1990 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Trospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F porin deficient mutants of E coli were more resistant than parental strains. Trospectomycin likely uses both porin and nonporin pathways to cross the outer membrane. An E coli strain effectively accumulated the drug anaerobically, explaining its anaerobic activity. An H influenzae strain accumulated trospectomycin at concentrations below those for which detectable uptake could be observed with E coli or S typhimurium strains, consistent with greater activity in Haemophilus species.