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Canadian Journal of Infectious Diseases
Volume 8, Issue 4, Pages 229-231
Brief Report

Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus

RK Chaudhary1 and E Burres2

1Laboratory for Viral Hepatitis, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, Canada
2Diagnostic Division, Abbott Laboratories, North Chicago, Illinois, USA

Received 19 August 1996; Accepted 15 January 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


One hundred and four clinical specimens from provincial public health laboratories were tested for antibody to hepatitis C virus (HCV) envelope protein (anti-E2). To evaluate the effect of hypervariability of E2 region on anti-E2 assay, 49 recombinant immunoblot assay (RIBA) 3.0 positive samples were genotyped. All 49 genotyped samples were positive for anti-E2. Eight of 12 (67%) indeterminate, HCV RNA positive samples were anti-E2 reactive. Nine of 30 (30%) indeterminate, HCV RNA negative samples were also positive for anti-E2. Anti-E2 was detected in two of 13 (15%) RIBA-negative and enzyme immunoassays-positive samples. Although small number of samples were tested, the results showed that it may be possible to resolve indeterminate samples with the anti-E2 assay.