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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 25, Issue 5, Pages 257-264
http://dx.doi.org/10.1155/2014/609016
Original Article

Nutritional Supplementation with the Mushroom Agaricus sylvaticus Reduces Oxidative Stress in Children with HIV

Marcela S Figueira,1 Luana A Sá,1 Amanda S Vasconcelos,1 Danilo R Moreira,1 Paula SOC Laurindo,1 Danielle RG Ribeiro,1 Rogério S Santos,1 Paulo Guzzo,2 Maria F Dolabela,3 and Sandro Percario1

1Oxidative Stress Research Laboratory – Institute of Biological Sciences – Federal University of Pará;, Brazil
2Specialized Reference Unit for Maternal & Adolescent Children (URE MIA);, Brazil
3Pharmacy Faculty, Institute of Health Sciences, Federal University of Pará – Belém, Pará, Brazil

Copyright © 2014 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

BACKGROUND: The involvement of free radicals and oxidative stress in HIV infection has been extensively studied, and the benefits of antioxidant supplementation in animal studies have been demonstrated. However, few studies have demonstrated a benefit in clinical studies.

OBJECTIVE: To verify the effects of dietary supplementation with Agaricus sylvaticus, a mushroom rich in antioxidants, on the oxidative profile of children born with HIV undergoing antiretroviral therapy.

DESIGN: The sample included 24 children (both boys and girls) between two and eight years of age, of whom 10 were HIV positive and received supplementation with Agaricus sylvaticus for a three-month period, and 14 were HIV negative and received no supplementation. At the beginning and conclusion of the study, thiobarbituric acid-reactive substances (TBARS), nitrite and nitrate (NN), Trolox equivalent antioxidant capacity, and the antioxidant capacity of inhibition of diphenyl-picrilhidrazil (DPPH) free radicals were analyzed.

RESULTS: Before supplementation, significantly higher values of TBARS and NN, but decreased values of DPPH, were observed in infected subjects when compared with HIV-negative subjects. After supplementation, a reduction of TBARS and NN values and an increase in DPPH and Trolox equivalent antioxidant capacity values were observed in HIV-positive subjects.

CONCLUSIONS: The results of the present study suggest the involvement of oxidative stress in HIV infection, with the participation of NN synthesis. Additionally, supplementation reversed oxidative alterations and improved antioxidant defense in infected individuals, and may become a complementary strategy in the treatment of these patients.