Molecular Characterization and Phylogenetic Analysis of Listeria monocytogenes Isolated from Milk and Milk Products in Kaduna, Nigeria

Table 2

Primer sequences used for the amplification of virulence-associated genes of Listeria monocytogenes.

Target gene	Primer sequence (5′-3′)	Product size (bp)
inl A	Fwd: 5′-AGATCTAGACCAAGTTACAACGCTTCAG-3′; Rev: 5′-TAA TAT CAT TTG CTG TTT TAT CTG TC-3′	255 bp
act A	Fwd: 5′′-ACG TGA AGT AAG TCACGT GAT ATT G 3′; Fwd: 5′′-ACG TGAAGTAAGCTCACGT GAT ATT G-3′	268 bp
prf A	Fwd: 5′-ACC GCT CAG AAA AGT TCT TC-3′; Rev: 5′-TCT TGT TCT ATT ATGTCT AGC-3′	1060 bp
iap	Fwd: 5′-ACA AGC TGC ACC TGT TGC AG-3′; Rev: 5-TGA CAG CGT TGT TAG TAG CA-3′	131 bp
hly A	Fwd: 5′-CCT AAG ACG CCA ATC GAA-3′; Rev: 5′-AAG CGC TTG CAA GTC CTC-3′	702 bp