Characterization of Clostridium difficile Strains in British Columbia, Canada: A Shift from NAP1 Majority (2008) to Novel Strain Types (2013) in One Region

Table 1

Characteristics of C. difficile () present in British Columbia in 2008 by NAP designation (number (%)).

	NAP1 ()	NAP2 ()	NAP4 ()	NAP6 ()	NAP7 ()	NAP10 ()	Other; ()	None; ()
Toxin gene presence
A+ B+, CDT+	144 (98.6)	1 (2.9)	0	0	0	0	0	17 (23.3)
A+ B+, CDT−	2 (1.4)	34 (97.1)	46 (100)	8 (100)	0	14 (100)	12 (100)	55 (75.3)
A− B+, CDT+	0	0	0	0	7 (100)	0	0	1 (1.4)
tcdC genotype
Wildtype	7 (4.8)	33 (94.3)	44 (95.7)	8 (100)	0	13 (92.9)	9 (75.0)	57 (78.1)
	137 (93.8)	2 (5.7)	0	0	0	0	0	5 (6.8)
	2 (1.4)	0	0	0	0	0	3 (25.0)	5 (6.8)
≥−37	0	0	0	0	7 (100)	0	0	6 (8.2)
≥+1	0	0	2 (4.3)	0	0	1 (7.1)	0	0

Overall distribution: 2 NAP3, 3 NAP5, 6 NAP11, and 1 NAP12. tcdC with an 18 bp deletion attributed to 2 NAP11 and 1 NAP12.
Isolates with pulsed field gel electrophoresis patterns not matching a known NAP type.
NAP: North American pulsotype.