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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2016 (2016), Article ID 8594107, 7 pages
http://dx.doi.org/10.1155/2016/8594107
Research Article

High-Throughput Analysis of the T Cell Receptor Beta Chain Repertoire in PBMCs from Chronic Hepatitis B Patients with HBeAg Seroconversion

1Hepatology and Cancer Biotherapy Ward, Beijing You’an Hospital, Capital Medical University, Beijing, China
2State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
3Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Received 27 March 2016; Revised 4 July 2016; Accepted 21 July 2016

Academic Editor: Roshanak Tolouei Semnani

Copyright © 2016 Yachao Qu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

T lymphocytes are the most important immune cells that affect both the development and treatment of hepatitis B. We used high-throughput sequencing to determine the diversity in the V and J regions of the TCRβ chain in 4 chronic hepatitis B patients before and after HBeAg seroconversion. Here, we demonstrate that the 4 patients expressed -4 at the highest frequencies of 10.6%, 9.2%, 17.5%, and 7.5%, and was the second most common, with frequencies of 7.8%, 6.7%, 5.3%, and 10.9%, respectively. No significant changes were observed following seroconversion. With regard to the Jβ gene, Jβ2-1 was the most commonly expressed in the 4 patients at frequencies of 5.8%, 6.5%, 11.3%, and 7.3%, respectively. Analysis of the V-J region genes revealed several differences, including significant increases in the expression levels of V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 and a decrease in that of V19-01-J2-3. These results illustrate the presence of biased TCRVβ and Jβ gene expression in the chronic hepatitis B patients. TRBVβ12-4, , Jβ2-1, V7-2-01-J2-1, V12-4-J1-1, and V28-1-J1-5 may be associated with the development and treatment of CHB.