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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2017 (2017), Article ID 3276240, 6 pages
https://doi.org/10.1155/2017/3276240
Research Article

First Insight into a Nationwide Genotypic Diversity of Mycobacterium tuberculosis among Previously Treated Pulmonary Tuberculosis Cases in Benin, West Africa

1Faculty of Health Sciences, Abomey-Calavi University, Cotonou, Benin
2National Reference Laboratory for Mycobacteria, Cotonou, Benin
3Department of Medical Microbiology & Parasitology, College of Medicine, University of Ibadan, Ibadan, Nigeria
4Department of Medical Microbiology & Parasitology, University College Hospital, Ibadan, Nigeria

Correspondence should be addressed to Dissou Affolabi; rf.oohay@uossid_ibaloffa

Received 17 March 2017; Revised 18 May 2017; Accepted 28 May 2017; Published 21 June 2017

Academic Editor: Cinzia Marianelli

Copyright © 2017 Dissou Affolabi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Molecular studies on tuberculosis (TB) are rare in low-resource countries like Benin, where data on molecular study on previously treated TB cases is unavailable. Materials and Methods. From January to December 2014, all smear- and culture-positive previously treated pulmonary TB patients from all TB clinics were systematically recruited. Drug susceptibility testing and spoligotyping were performed on all isolates. Results. Of the 100 patients recruited, 71 (71.0%) were relapse cases and 24 (24.0%) were failure cases, while 5 (5.0%) were default cases. Resistance rate to any first-line drug was 40.0%, while 12.0% of strains were multidrug-resistant (MDR) and no strain was extensively drug-resistant (XDR). A total of 40 distinct spoligotypes were found to be corresponding to a genotypic diversity of 40.0%. ST61 was the most predominant spoligotype with prevalence of 33.0%. In all, 31 single spoligotypes and nine clusters were observed with 2 to 33 strains per cluster giving a clustering rate of 69.0%. Euro-American (Lineage 4) was the most prevalent lineage (74.0%) and Lineage 2 was associated with resistance to streptomycin. Conclusion. This first insight into genetic diversity of previously treated pulmonary TB patients in Benin showed a relatively high genetic diversity of Mycobacterium tuberculosis.