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Canadian Journal of Infectious Diseases and Medical Microbiology
Volume 2017, Article ID 6094587, 7 pages
https://doi.org/10.1155/2017/6094587
Research Article

Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México

1Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, Mexico
2Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuaria, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Ex-Hda. de Aquetzalpa, 43600 Tulancingo, HGO, Mexico
3Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Panamericana Toluca-Atlacomulco, 50200 Toluca, MEX, Mexico
4Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, 04960 Ciudad de México, Mexico

Correspondence should be addressed to Ninfa Ramírez Durán; xm.xemeau@dzerimarn

Received 9 March 2017; Revised 12 May 2017; Accepted 21 May 2017; Published 14 June 2017

Academic Editor: Nahuel Fittipaldi

Copyright © 2017 Adrian Zaragoza Bastida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of M. bovis in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as M. neoaurum (six strains), M. parafortuitum (four strains), M. moriokaense (two strains), and M. confluentis (one strain). Except M. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.