Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México

Table 3

Comparison of 16S rRNA gene sequences of strains isolated from cattle with those documented in GenBank, using BLAST and EzTaxon.

Strain	Origin of the herd	Culture medium	Amplified fragment size (bp)	Similarity (Blast)	%	Similarity (EzTaxon)	%
1-AZ	2	Middlebrook	1428	M. neoaurum	98	M. neoaurum	98.3
2-AZ	2	Stonebrink	1408	M. neoaurum	99	M. neoaurum	99.1
3-AZ	2	Stonebrink	1428	M. neoaurum	98	M. neoaurum	98.2
5-AZ	3	Stonebrink	1416	M. neoaurum	99	M. neoaurum	99.2
8-AZ	4	Middlebrook	1415	M. neoaurum	99	M. neoaurum	99.0
12-AZ	2	Middlebrook	1415	M. neoaurum	99	M. neoaurum	99.4
4-AZ	4	Middlebrook-P	1420	M. parafortuitum	99	M. parafortuitum	98.2
9-AZ	3	Middlebrook	1415	M. parafortuitum	99	M. parafortuitum	98.9
10-AZ	4	Stonebrink	1411	M. parafortuitum	99	M. parafortuitum	98.4
11-AZ	3	Stonebrink	1414	M. parafortuitum	99	M. parafortuitum	98.2
6-AZ	2	Stonebrink	1455	M. moriokaense	99	M. moriokaense	98.2
13-AZ	4	Stonebrink	1417	M. moriokaense	98	M. moriokaense	98.2
7-AZ	2	Middlebrook-P	1420	M. confluentis	99	M. confluentis	99.1

2: Zacazonapan Holstein-F; 3: Zacazonapan Holstein-F; 4: Zacazonapan Holstein-F.