Table 2: Performance analysis of different tests with species-specific PCR as internal control.

VariablesICT Malaria ComboFirst Response MalariaMicroscopyAutomated hemoanalyzer
(Sysmex XE-2100)
PCR

True positive109108126126127
True negative150150150150150
False positive87000
False negative1110221
Negative Predictive Value (95% CI)93.16 (90.16–96.16)93.75 (90.85–96.65)98.68 (96.31–99.60)98.68 (96.31–99.60)99.33 (99.24–99.42)
Positive predictive value (95% CI)93.16 (90.16–96.16)93.90 (91.10–96.70)99.20 (98.20–100.20)99.20 (98.20–100.20)100
Sensitivity (95% CI)90.83 (86.83–94.83)91.52 (87.52–95.52)98.41 (96.40–100.40)98.41 (96.40–100.40)99.21 (98.20–100.20)
Specificity (95% CI)94.90 (92.30–97.50)95.54 (93.14–97.94)100100100
Accuracy (95% CI)93.16 (90.16–96.16)92.80 (89.7–97.7)99.28 (97.00–101.00)99.28 (97.00–101.00)99.64 (99.57–99.71)
Youden’s J- index0.860.870.980.980.99