Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests

Table 2

Performance analysis of different tests with species-specific PCR as internal control.

Variables	ICT Malaria Combo	First Response Malaria	Microscopy	Automated hemoanalyzer; (Sysmex XE-2100)	PCR
True positive	109	108	126	126	127
True negative	150	150	150	150	150
False positive	8	7	0	0	0
False negative	11	10	2	2	1
Negative Predictive Value (95% CI)	93.16 (90.16–96.16)	93.75 (90.85–96.65)	98.68 (96.31–99.60)	98.68 (96.31–99.60)	99.33 (99.24–99.42)
Positive predictive value (95% CI)	93.16 (90.16–96.16)	93.90 (91.10–96.70)	99.20 (98.20–100.20)	99.20 (98.20–100.20)	100
Sensitivity (95% CI)	90.83 (86.83–94.83)	91.52 (87.52–95.52)	98.41 (96.40–100.40)	98.41 (96.40–100.40)	99.21 (98.20–100.20)
Specificity (95% CI)	94.90 (92.30–97.50)	95.54 (93.14–97.94)	100	100	100
Accuracy (95% CI)	93.16 (90.16–96.16)	92.80 (89.7–97.7)	99.28 (97.00–101.00)	99.28 (97.00–101.00)	99.64 (99.57–99.71)
Youden’s J- index	0.86	0.87	0.98	0.98	0.99