Development of IgY-Based Sandwich ELISA as a Robust Tool for Rapid Detection and Discrimination of Toxigenic Vibrio cholerae

Table 4

The result of analytical sensitivity and specificity of the developed ELISAs in spiked stool sample.

α-OmpW			α-CtxB
Spiked conc. (Cfu·mL^−1)	Final conc. (cfu·mL^−1)	OD	Spiked conc. (pg·mL^−1)	Final conc. (pg·mL^−1)	OD
7.5 × 10^7	3.8 × 10^7	2.86	500	250	1.73
7.5 × 10^6	3.8 × 10^6	1.92	250	125	1.02
7.5 × 10^5	3.8 × 10^5	1.25	125	62.5	0.45
7.5 × 10^4	3.8 × 10^4	0.69	62.5	31.3	0.21
7.5 × 10^3	3.8 × 10^3	0.41	31.3	15.6	0.14
7.5 × 10^2	3.8 × 10^2	0.18	15.6	7.8	0.08
7.5 × 10^1	3.8 × 10^1	0.07	7.8	3.9	0.06
0 (negative control)	0	0.08	0 (negative control)	0	0.06

Final concentration is calculated by considering the dilution factor used for preparation of cleared sample.