Research Article

Clinical, Serological, and Molecular Observations from a Case Series Study during the Asian Lineage Zika Virus Outbreak in Grenada during 2016

Table 3

Details of the rRT-PCR assay used in testing for CHIKV, DENV, and ZIKV, including the genome regions targeted, the fluorescent dyes, and the quencher.

ā€‰AssayTargetFluorescent dyeQuencher

(1)Chikungunya (CHIKV)NP 3 regionDFOBHQ
(2)Dengue (DENV)3ā€² UT conserved regionFAMBHQ
(3)Zika (ZIKV)NS 5 regionTexas redBHQ
(4)Internal control (IC)Synthetic oligoATTO 647BHQ