Canadian Journal of Infectious Diseases and Medical Microbiology / 2018 / Article / Tab 3 / Research Article
Development of Microsatellite Markers for the Nipa Palm Hispid Beetle, Octodonta nipae (Maulik) Table 3 Characteristics of 9 microsatellite loci isolated from Octodonta nipae .
Locus Primer sequences (5′-3′) Repeat motif T A (°C)Range (bp) N A H e H o PIC HWE ZZ FZ ZZ FZ ZZ FZ ZZ FZ On1 F: AACATAGAATCGACATCATGCGT R: GATTTACAGAACGGAGTTCATGC (TA)8 47.6 96–98 2 0.083 0.344 0.083 0.250 0.077 0.275 — 0.404 On2 F: AGTTGCATAACTTCCGAAATGAA R: TCTAGGAATCAGCTCTGCCATTA (GT)9 52.4 99–103 3 0.681 0.591 0.667 0.250 0.579 0.501 0.398 0.002 On3 F: GCTTTGCAACAACAAAGAAGAAT R: TCAAAAGAAAGGAAAAAGTTCCC (CA)10 50.9 97–105 3 0.678 0.359 0.750 0.417 0.584 0.307 0.015 1.000 On4 F: AGACGTCAAGTTTTAACACGCAT R: TATAGACGGAGAACTGGCAACAT (AT)6 53.7 113–117 2 0.159 0.000 0.167 0.000 0.141 0.001 1.000 — On5 F: TGCTTAGATTTAGCACCAAGCTG R: TTAAATTCGAACCAAGCTGGTAG (GT)8 53.1 131–137 3 0.562 0.522 0.583 0.333 0.432 0.375 1.000 0.282 On6 F: AGCTAATTGCGGCTGATTAGATA R: GCGGTAAATATGTCAAAAGCAAG (AT)6 50.2 159–161 2 0.159 0.000 0.167 0.000 0.141 0.001 1.000 — On7 F: ATGTGTACCCTGAAAGAGGGTTT R: GAAAAATTTGAGAAGCTTCGGTT (CT)7 51.3 155–163 3 0.663 0.453 0.417 0.417 0.561 0.369 0.065 1.000 On8 F: AATGAAAAATTGACTTGGCAGAA R: CCGGCGTACATAACCTACTTTTT (AAG)5 51 121–127 3 0.344 0.431 0.417 0.583 0.275 0.328 0.001 1.000 On9 F: AACTTAAACCAGAATCGGCTCTC R: ATGATGTCGGACGATTCTCTTC (CAA)5 55 138–141 2 0.663 0.163 0.167 0.167 0.561 0.150 1.000 0.487
T A , primer annealing temperature; range, size range of alleles in base pairs; N A , number of alleles per locus; H e , expected heterozygosity; H o , observed heterozygosity; PIC, polymorphic information content; HWE, Hardy–Weinberg equilibrium.