Research Article

A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate

Figure 1

Cloning of acr gene. (a) Restriction digestion of clones: Lane 1: 100 bp ladder; Lane 2: Clone #3 digested with NdeI and XhoI; Lane 3: Clone #6 digested with NdeI and XhoI; Lane 4: pET28a control digested with NdeI and XhoI. (b) PCR of recombinant plasmids: Lanes 1 to 3: Clones #1, 3, and 5; Lane 4: 100 bp ladder; Lanes 5 and 6: Clones 6 and 7; Lane 7: negative control (water); Lane 8: positive control (genomic DNA).
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