A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate

Figure 4

CD of (H) samples: (H) samples were checked using the Jasco spectropolarimeter (Far-UV CD) 190 nm to 260 nm at 25°C and 37°C (without pre-heat treatment) and 37°C, 60°C and 70°C (with pre-heat treatment for 15 mins). (a) Far-UV CD analysis of (H) samples at 25°C without pre-heat treatment. (b) Far-UV CD analysis of (H) samples at 37°C without pre-heat treatment. (c) Far-UV CD analysis of (H) samples after pre-heat treatment for 15 mins at 37°C. (d) Far-UV CD analysis of (H) samples after pre-heat treatment for 15 mins at 60°C. (e) Far-UV CD analysis of (H) samples after pre-heat treatment for 15 mins at 70°C. (f) Overlay of Far-UV CD analysis of (H) samples: 25°C without pre-heat treatment (indicated in black); 37°C without pre-heat treatment (indicated in purple); 37°C with pre-heat treatment (indicated in blue); 60°C with pre-heat treatment (indicated in green); and 70°C with pre-heat treatment (indicated in red). (g) Far-UV CD analysis of (G) samples at 37°C without pre-heat treatment. (h) Far-UV CD analysis of (G) samples at 37°C with pre-heat treatment. (i) Far-UV CD analysis of (G) samples with pre-heat treatment at 60°C. (j) Far-UV CD analysis of (G) samples with pre-heat treatment at 70°C. (k) Overlay of Far-UV CD analysis of (G) samples: 37°C without pre-heat treatment (indicated in red); 37°C with pre-heat treatment (indicated in blue); 60°C with pre-heat treatment (indicated in green); 70°C with pre-heat treatment (indicated in brown).