A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate

Table 1

Oligomer ratio calculation: oligomer sizes are calculated, using BSA monomer, dimer, and trimer as a reference (66, 132, and 198 kDa), and the log of molecular weight plotted versus the distance travelled in centimeter. From this calculation, molecular weights are extrapolated for the different bands viewed in (H) (bands 1, 2, 3, and 4) and (G) samples (bands 1, 2, 3, 4, and 5) using the equation used in Figure 2(h) (y = 0.294x + 2.999). The proportion of bands was estimated using ImageJ software. For distance migrated, the error was ±0.05 cm, and for estimating the proportion, an assumption of ±10% was made.

BSA (mol. wt.)	Log mol wt.	Distance (cm)
198	2.29	2.4 ± 0.24
132	2.12	3 ± 0.03		Monomer
66	1.82	4 ± 0.14		18
			Size (kDa)	Average oligomer size	% Oligomer fraction of total amount of protein
(H) band 1	2.21 ± 0.04	2.7 ± 0.05	162 ± 15	8.84	70 ± 7
(H) band 2	2.264 ± 0.04	2.5 ± 0.05	184.93 ± 15	10	10 ± 1
(H) band 3	2.29 ± 0.03	2.35 ± 0.05	204 ± 18	11.16	10 ± 1
(H) band 4	2.35 ± 0.02	2.2 ± 0.05	229.8 ± 18	12.48	10 ± 1
				Ratio of 10 mers to 12 mers	30 ± 3
				9 mers	70 ± 7
(G) band 1		Same as (H)		9	15 ± 1.5
(G) band 2				10	15 ± 1.5
(G) band 3				11	15 ± 1.5
(G) band 4				12.48	15 ± 1.5
(G) band 5	2.7	1.2	436	24	40 ± 4
				Ratio of 10 mers to 24 mers	85 ± 8.5
				9 mers	15 ± 1.5