A New Functional Model for Prediction of Chaperone Activity of the Recombinant M. tb Acr (α-Crystallin) Using Insulin as Substrate

Table 6

A comparison of (H) and (G) samples in terms of 4 secondary structures to achieve 95% inhibition of insulin aggregation. The number of molecules of 4 secondary structures required to achieve 95% inhibition was compared for (H) and (G) samples and the total number of molecules (mass balance) compared with the theoretical number of molecules expected by Native-PAGE and the amount used for assay.

Secondary conformations and other parameters	Molecules in (H) samples	Molecules in (G) samples	Ratios (G)/(H)
β-sheets	1.1 × 10^13	6.1 × 10^13	5.55
α-helices	1.2 × 10^14	1.29 × 10^13	0.12
Random coils	1.9 × 10^14	4.63 × 10^13	0.08
Total molecules	4.81 × 10^14	1.33 × 10^14	0.28
Actual expected by Native-PAGE	4.54 × 10^14	1.47 × 10^14	0.32
Mass balance	106%	91%	—