Seasonal and Gender Differences in Presence of Rickettsia felis and Blood meals Provide Additional Evidence of a Vector Role for Mosquitoes

Table 1

Primers and probes used in the PCR reactions in this study.

PCR	Primer/probe nucleotides	Amplicon	Reference
HMBS FRET-qPCR	Forward primer: 5′-tacctgactggaggagtctggagtct-3′	286 bp	[13]
	Downstream primer: 5′-gccaggctgatgcccaggttct-3′
	Anchor probe: 5′-agcahgaagatggyccwga-6-FAM-3′
	Reporter probe: 5′-LCRed640-gatgayccacagctggtrg-phos-3′
Rickettsia gltA FRET-qPCR	Up primer: 5′-ttrcaaatagcaatagaacttgaagct-3′	170 bp	[5]
	Reverse primer: 5′-agcaagaaccgtaggctggat-3′
	Anchor probe: 5′-atcgctcttaaagatgaatattttattgag-6-FAM-3′
	Reporter probe: 5′-LCRed640-gaaaattatatccaaatgttgatttttattc-phos-3′
Rickettsia gltA nested-PCR	Out-up primer: 5′-agtaaatccaataataaaaaatgckcttaata-3′	446 bp	[5]
	Out-down primer: 5′-cttaaagatgaatattttattgagagaaaat-3′
	In-up primer: 5′atgagcagaatgcttctacttcaaca-3′	353 bp
	In-down primer: 5′-ttrcaaatagcaatagaacttgaagct-3′
Mosquito COI PCR	LCO1490: 5′-ggtcaacaaatcataaagatattgg-3′	710 bp	[14]
	HC02198: 5′-taaacttcagggtgaccaaaaaatca-3′