Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis

Table 3

Comparison of the results between EvaGreen MCA and conventional culture method.

Bacterial pathogens	EvaGreen MCA	Conventional culture method		Sensitivity (%)	Specificity (%)	Agreement (%)	Kappa
		Positive	Negative
A. baumannii	Positive	30	0	100	100	100	1.00
	Negative	0	127
E. coli	Positive	11	0	91.7	100	99.4	0.95
	Negative	1	145
K. pneumoniae	Positive	18	0	100	100	100	1.00
	Negative	0	139
H. influenzae	Positive	4	0	80	100	99.4	0.89
	Negative	1	152
S. pneumoniae	Positive	2	0	100	100	100	1.00
	Negative	0	155
S. aureus	Positive	13	0	100	100	100	1.00
	Negative	0	144
P. aeruginosa	Positive	17	0	100	100	100	1.00
	Negative	0	140
M. tuberculosis	Positive	9	0	90	100	99.4	0.94
	Negative	1	147