Research Article
Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria
Table 1
Primer sequence/PCR conditions for the ESBL resistance genotyping [
12,
40].
| | Genes | Primer sequences (5′-3′) | Annealing temp. (°C) | No. of cycles (2–4) | Amplicon size (bp) |
| | TEM | F: CATTTCCGTGTCGCCCTTATTC
R: CGTTCATCCATAGTTGCCTGAC | 60 | 30 | 800 | | SHV | F: AGCCGCTTGAGCAAATTAAAC
R: ATCCCGCAGATAAATCACCAC | 60 | 30 | 713 | | OXA-1-like | F: GGCACCAGATTCAACTTTCAAG
R: GACCCCAAGTTTCCTGTAAGTG | 60 | 30 | 564 | | CTX-M-1 | F: TTAGGAAATGTGCCGCTGTA
R: CGATATCGTTGGTGGTACCAT | 60 | 30 | 688 | | CTX-M-2 | F: CGTTAACGGCACGATGAC
R: CGATATCGTTGGTGGTACCAT | 60 | 30 | 404 | | CTX-M-9 | F: TCAAGCCTGCCGATCTGGT
R: TGATTCTCGCCGCTGAAG | 60 | 30 | 561 | | GES 1–9, 11 | F: AGTCGGCTAGACCGGAAAG
R: TTTGTCCGTGCTCAGGAT | 60 | 30 | 399 | | PER 1,3 | F: GCTCCGATAATGAAAGCGT
R: TTCGGCTTGACTCGGCTGA | 60 | 30 | 520 | | VEB 1–6 | F: CATTTCCCGATGCAAAGCGT
R: CGAAGTTTCTTTGGACTCTG | 60 | 30 | 648 |
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