Comparative Study of Clostridium difficile Clinical Detection Methods in Patients with Diarrhoea

Table 1

Primers used in the present analysis.

Gene target	Primer name	Sequence (5′ to 3′)	Primer concentration (μM)	Amplicon size (bp)
tcdA	tcdA-F3345	GCATGATAAGGCAACTTCAGTGGTA	0.6	629
	tcdA-R3969	AGTTCCTCCTGCTCCATCAAATG	0.6
tcdB	tcdB-F5670	CCAAARTGGAGTGTTACAAACAGGTG	0.4	410
	tcdB-R6079A	GCATTTCTCCATTCTCAGCAAAGTA	0.2
	tcdB-R6079B	GCATTTCTCCGTTTTCAGCAAAGTA	0.2
cdtA	cdtA-F739A	GGGAAGCACTATATTAAAGCAGAAGC	0.05	221
	cdtA-F739B	GGGAAACATTATATTAAAGCAGAAGC	0.05
	cdtA-R958	CTGGGTTAGGATTATTTACTGGACCA	0.1
ctdB	ctdB-F617	TTGACCCAAAGTTGATGTCTGATTG	0.1	262
	cdtB-R878	CGGATCTCTTGCTTCAGTCTTTATAG	0.1
16S rDNA	PS13	GGAGGCAGCAGTGGGGAATA	0.05	1062
	PS14	TGACGGGCGGTGTGTACAAG	0.05