Transcriptional Profile of Helicobacter pylori Virulence Genes in Patients with Gastritis and Gastric Cancer

Table 1

Primers used in this study.

Specific primers	Sequence	Annealing Tm, °C	Product size, bp	Reference
16s rDNA H. pylori F; 16s rDNA H. pylori R	CTGGAGAGACTAAGCCCTCC; AGGATCAAGGTTTAAGGATT	50	446	This study
cagA F; cagA R	TGACCAACAACCACAAACCG; TCAGGATCGTATGAAGCGACAG	57	108	This study
cagA EPIYA-C F; cagA EPIYA-C R	AAGAAAGCAGGACAAGCAGC; CTAACCGATCGCCCTACCTT	55	188	This study
cagT F; cagT R	AGGGTGTGGTGATGATAGCG; TGCTTGTTGTTTGCTCCACT	55	154	This study
cagE F; cagE R	GAATGGAGCGAGCGATGAAA; TAGGAATTTGCAGCGCTCAC	56	163	This study
cagY F; cagY R	AGTTCAAGTGGCGCTAGATTG; ACAAGCCTTTCAAGCATTCGT	57	200	This study
vacA s1/s2 F; vacA s1/s2 R	ATGGAAATACAACAAACACAC; CTGCTTGAATGCGCCAAAC	55	259/286	Atherton et al. [40]
vacA m1/m2 F; vacA m1/m2 R	TGGATAGTGCGACTGGGTTT; TCCATGCGGTTGTTGTTGTT	54	205/220	This study
iceA1 F; iceA1 R	GTGTTTTTAACCAAAGTATC; CTATAGCCASTYTCTTTGCA	45	247	van Doorn et al. [41]
iceA2 F; iceA2 R	GTTGGGTATATCACAATTTAT; TTRCCCTATTTTCTAGTAGGT	47	229/234	van Doorn et al. [41]
hopQI F; hopQI R	ACGAACGCGCAAAAACTTTA; TTGCCATTCTCATCGGTGTA	55	187	Sicinschi et al. [42]
hopQII F; hopQII R	ACAGCCACTCCAATCCAGAA; AACCCCACCGTGGATTTTAG	55	160	Sicinschi et al. [42]
babA2 F; babA2 R	CAATGCGGTGCGTGAAAATC; ATACCCTGGCTCGTTGTTGA	57	205	This study
babB F; babB R	CAATTCCCCGGCGTATCAAG; ATTGCAAGTGATGGTCGTCG	56	175	This study
sabA F; sabA R	TCTCTCGCTTGCGGTATCAT; AGCTCAATGTTGTTGGCGTT	56	204	This study
sabB F; sabB R	GCATTCAAACGGCGAACAAC; TCCTGTGCAGTTCCCATCTT	56	248	This study
alpA F; alpA R	CGCTCCTATCAAAACCGCTC; TTCCCGTCCAACTTACCGAA	55	185	This study
alpB F; alpB R	TCAACTTGCGAGCAGACCTA; AGCCATAGACCCCATACACG	57	218	This study
oipA F; oipA R	CTCCACGCTGAAAGGAATGG; CCATTTCCTGCGAATCGGTT	55	233	This study

The primers yield a fragment of 229 or 334 bp depending on the presence of a repetitive sequence of 105 nucleotides in some iceA2 alleles [40].