Evaluation of Human Respiratory Syncytial Virus and Human Parainfluenza Virus Type 3 among Hospitalized Children in Northwest of Iran

Table 1

Sequences of oligonucleotides used for detection of RSV and HPIV-3.

Primers name	Target gene	Sequence (5′ to 3′)	Product size (bp)	Reference
Seminested RT-PCR for RSV	N	Forward (1): GGAACAAGTTGTTGAGGTTTATGA ATATGC	210	[14]
		Reverse (2): TTCTGCTGTCAAGTCTAGTACACT GTAGT
		Reverse (3): TTCTGCTGTCAAGTCTAGTACACTGTA GT	180
Heminested RT-PCR for HPIV-3	HN	Forward (1): CTCGACGTTGTCAGGATATAG	189
		Reverse (2): CTTTGGGACTTGAACACAGTT
		Reverse (3): GCTAGAGAACATGACTTCC	145

Abbreviations. N: nucleocapsid; HN: hemagglutinin-neuraminidase.