Distinct Patterns of Host Adherence by Neisseria gonorrhoeae Isolated from Experimental Gonorrhea

Figure 1

Analysis of the pili, Opa protein, and LOS phenotypes of reisolates. (a) Whole cell lysates of reisolates were subjected to SDS-PAGE and were immunoblotted with anti-pilus monoclonal antibody MCO2. (b) The expression of the Opa protein was detected with monoclonal antibody 4B12, which reacts with all of the Opa proteins expressed by the strain MS11mk. (c) The LOS preparations were analyzed by SDS-PAGE and then immunoblotting with αLOSa and αLOSb. Autoradiography was used to detect the LOS labelled in vitro. Lanes 1 to 6 represent G29, 4R1, SA, 7uB, 6uF, and V3, respectively. The molecular mass markers (in kilodaltons) are indicated on the left side of the panels.

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