Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis

Table 1

Oligonucleotide primers used in the present study.

	Target gene	Species	Size (bp)	Oligonucleotide sequence (5′- 3′)	Ref
1^st mPCR	IS901	M. avium subsp. avium	753	F: GAACGCTGCTCTAAGGACCTGTTG	[21]
				R: GGAAGGGTGATTATCTGGCCTGC
	rv0577	MTB complex	705	F: ATGCCCAAGAGAAGCGAATACA	[22]
				R: AATGTCAGCCGGTTCCGCAA
	16S rRNA	All mycobacterial species	484	F: ATAAGCCTGGGAAACTGGGT	[21]
				R: CACGCTCACAGTTAAGCCGT
	RD9	MTB	358	F: GTGTAGGTCAGCCCCATCC	[22]
				R: GTGTGGATTCCGTGGGCG
	IS1311	M. avium complex	282	F: CATGAACGGAGCGCATCAC	In this study
				R: ATCGGCGCAAATCCGGGA
	DT1	M. intracellulare	106	F: AAGGTGAGCCCAGCTTTGAACTCCA	[22]
				R: GCGCTTCATTCGCGATCATCAGGTG
2^nd mPCR	Transferase	M. kansasii	582	F: ACTTCTTTCGTCGTCGCGAC	In this study
				R: GCAGAAGCACAGATCCCAACC
	mass_3210	M. abscessus/M. massiliense	310/1145	F: GCTTGTTCCCGGTGCCACAC	[22]
				R: GGAGCGCGATGCGTCAGGAC
	SOD	M. fortuitum	275	F: CCAAGCTCGATGAGGCGCGG	[27]
				R: CCGATCGCCCAGGCTGTCGT
Sequencing	rpoB		950	F: TCGACGAGTGCAAAGACAAGGAC	In this study
				R: CTGGTGCCGAAGAACTCCTTGA
	hsp65		640	F: AGGGTATGCGGTTCGACAAG	In this study
				R: GACGATGCCCTCCTCCACGG
	ITS		420	F: GTGCGGCTGGATCACCTCCT	In this study
				R: GGTTGACAGCTCCCCGAGGCA

MTB, M. tuberculosis; SOD, superoxide dismutase; rRNA, ribosomal RNA; ITS, internal transcribed spacer. 16S rRNA primers were used for the first mPCR and sequencing.