Identification of Nontuberculous Mycobacteria in Patients with Pulmonary Diseases in Gyeongnam, Korea, Using Multiplex PCR and Multigene Sequence-Based Analysis
Table 3
Results of multigene sequence-based analysis by comparing the four target gene sequences against the reference strains and those obtained from the NCBI database.
16S rRNA
rpoB
hsp65
ITS
Strains
Strains
Percent homology (%)
Strains
Percent homology (%)
Strains
Percent homology (%)
Strains
Percent homology (%)
CI-1
M. chimaera (CP023151.1)
100
M. chimaera (CP023151.1)
99
M. chimaera (CP023151.1)
100
M. chimaera (CP023151.1)
99.7
M. intracellulare (CP023149.1)
100
M. intracellulare (CP003324.1)
96.7
M. intracellulare (CP003347.1)
100
M. paraintracellulare (AP022597.1)
99.7
CI-2
M. gordonae (MK630261.1)
99.8
M. gordonae (LQOY01000155.1)
99.9
M. gordonae (LQOY01000150.1)
99.7
M. gordonae (LR031451.1)
99.7
M. paragordonae (MF437319.1)
99.8
M. paragordonae (CP025546.1)
96.1
M. paragordonae (CP025546.1)
95.3
M. paragordonae (CP025546.1)
98.3
CI-3
M. gordonae (MK630261.1)
100
M. gordonae (LQOY01000155.1)
100
M. gordonae (LQOY01000150.1)
98.1
M. gordonae (LR031451.1)
99.7
M. paragordonae (MF437319.1)
100
M. paragordonae (CP025546.1)
96.2
M. paragordonae (CP025546.1)
93.9
M. paragordonae (CP025546.1)
98.3
CI-4
M. colombiense (CP020821.1)
100
M. paraintracellulare (AP022597.1)
97.1
M. colombiense (CP020821.1)
99
M. colombiense (CP020821.1)
98.4
M. intracellulare (KM096752.1)
100
M. colombiense (CP020821.1)
96.6
M. bouchedurhonense (EU239790.1)
98.8
M. mantenii (AP022590.1)
96.8
CI-5
M. chimaera (CP023151.1)
99.3
M. chimaera (CP023151.1)
100
M. chimaera (CP023151.1)
100
M. chimaera (CP023151.1)
99.7
M. intracellulare (CP023149.1)
99.3
M. intracellulare (CP003324.1)
97.7
M. intracellulare (CP003324.1)
100
M. intracellulare (CP023149.1)
99.7
CI-6
M. chimaera (CP045963.1)
100
M. chimaera (CP022223.1)
100
M. chimaera (CP045963.1)
99.8
M. chimaera (CP045963.1)
99.7
M. intracellulare (KP736000.1)
100
M. intracellulare (CP003324.1)
97.5
M. paraintracellulare (AP022597.1)
99.8
M. marseillense (AP022584.1)
95.2
CI-7
M. gordonae (MK630261.1)
100
M. gordonae (LQOY01000155.1)
99.9
M. gordonae (LQOY01000150.1)
98.4
M. paragordonae (CP025546.1)
98.6
M. paragordonae (MF437319.1)
100
M. paragordonae (CP025546.1)
96.3
M. paragordonae (CP025546.1)
95.7
M. gordonae (HG313849.1)
98.5
CI-8
M. chimaera (CP045963.1)
100
M. chimaera (CP022223.1)
100
M. chimaera (CP045963.1)
100
M. chimaera (CP045963.1)
100
M. intracellulare (KP736000.1)
100
M. intracellulare (CP003324.1)
97.5
M. paraintracellulare (AP022597.1)
100
M. marseillense (AP022584.1)
95.4
CI-9
M. persicum (MN936127.1)
99.2
M. kansasii (KY933082.1)
100
M. persicum (MWKZ01000001.1)
99.1
M. gordonae (CP059165.1)
93.1
M. kansasii (KP736051.1)
99.2
M. persicum (MWKZ01000001.1)
99.8
M. kansasii (CP006835.1)
97.2
M. kansasii (CP006835.1)
93.1
M. persicum (MWKZ01000001.1)
90.9
CI-10
M. mageritense (AP022567.1)
99.6
M. mageritense (AP022567.1)
99.8
M. mageritense (AP022567.1)
97.6
M. mageritense (AP022567.1)
94
M. peregrinum (AF537362.1)
99.6
M. goodii (AY262736.1)
96.1
M. dioxanotrophicus (CP020809.1)
97.6
M. fortuitum (KR995170.1)
87.6
CI-11
M. marseillense (AP022584.1)
99.8
M. chimaera (CP045963.1)
99.7
M. chimaera (CP045963.1)
99.8
M. intracellulare (CP003347.1)
100
M. intracellulare (KR856204.1)
99.8
M. intracellulare (CP003324.1)
97.4
M. paraintracellulare (AP022597.1)
99.8
M. marseillense (AP022584.1)
98.6
M. chimaera (CP045963.1)
99.5
M. marseillense (CP023147.1)
95
M. timonense (EU239792.1)
99.1
M. chimaera (CP023151.1)
98
CI-12
M. gordonae (MK630261.1)
100
M. gordonae (LQOY01000155.1)
100
M. gordonae (LQOY01000150.1)
100
M. gordonae (LR031451.1)
100
M. paragordonae (MF437319.1)
100
M. paragordonae (CP025546.1)
96.2
M. paragordonae (CP025546.1)
95
M. paragordonae (CP025546.1)
98.7
CI-13
M. chimaera (CP022597.1)
100
M. chimaera (CP045963.1)
99.3
M. chimaera (CP023151.1)
99.5
M. intracellulare (CP003347.1)
99.5
M. colombiense (CP020821.1)
100
M. intracellulare (CP003324.1)
97.1
M. intracellulare (CP003347.1)
99.5
M. chimaera (CP023151.1)
99
ND#1
M. kansasii (KF687952.1)
100
M. kansasii (KY933082.1)
95.2
M. chimaera (CP045963.1)
100
M. basiliense (LR130759.1)
93
M. riyadhense (NR_044449.1)
100
M. chimaera (CP045963.1)
93.7
M. paraintracellulare (AP022597.1)
100
M. marinum (CP000854.1)
89.9
M. haemophilum (MN535540.1)
99.7
M. mantenii (AP022590.1)
93
M. timonense (EU239792.1)
99.1
M. kansasii (CP019888.1)
89.6
ND#2
M. marseillense (AP022584.1)
100
M. colombiense (CP020821.1)
97.1
M. colombiense (CP020821.1)
97.9
M. mantenii (AP022590.1)
96.1
M. intracellulare (KR856204.1)
100
M. paraintracellulare (AP022597.1)
96.6
M. marseillense (AP022584.1)
97.9
M. chimaera (CP022223.1)
96.1
M. colombiense (CP020821.1)
99.7
M. marseillense (AP022584.1)
95.2
M. chimaera (CP023151.1)
97.6
M. colombiense (CP020821.1)
95.4
ND#3
M. kansasii (KF687952.1)
100
M. kansasii (KY933082.1)
95.2
M. chimaera (CP045963.1)
100
M. basiliense (LR130759.1)
92.6
M. riyadhense (NR_044449.1)
100
M. chimaera (CP045963.1)
93.7
M. paraintracellulare (AP022597.1)
100
M. marinum (CP000854.1)
90
M. haemophilum (MN535540.1)
99.7
M. mantenii (AP022590.1)
93
M. timonense (EU239792.1)
99.3
M. shotsii (AP022572.1)
89.3
Sixteen clinical isolates not determined by two-step mPCR were randomly numbered and named as CI-1–CI-13 and ND#1–ND#3.
