Comparison between Whatman FTA Elute Cards and Conventional Swab for the Detection of Pathogenic Enteric Bacteria Using an RT-qPCR Assay

Figure 7

CT values of the Staphylococcus aureus gene in Whatman FTA Elute cards are higher than those on swabs. (a) Expression of the Staphylococcus aureus gene FemB in samples collected using Whatman FTA Elute cards detected by RT-qPCR (n = 6, , , and ). (b) Expression of the Staphylococcus aureus gene FemB in samples collected using swabs detected by RT-qPCR (n = 6, and ). (c) Expression level of the FemB gene in an environmental strain of Staphylococcus aureus samples on Whatman FTA cards and swabs detected by RT-qPCR (n = 6, and ). RT-qPCR: reverse transcription-quantitative PCR; FTA: Flinders Technology Associates.

(a)

(b)

(c)