Comparison between Whatman FTA Elute Cards and Conventional Swab for the Detection of Pathogenic Enteric Bacteria Using an RT-qPCR Assay

Table 1

Primers used in the study.

Bacteria	Gene name		Sequence (5′-3′)	Amplicon size expected (bp)
Escherichia coli O157:H7	Shiga toxins (stx1)	Forward	TTTGTYACTGTSACAGCWGAAGCYTTACG	132
		Reverse	CCCCAGTTCARWGTRAGRTCMACRTC
		Probe-FAM	CTGGATGATCTCAGTGGGCGTTCTTATGTA A
	Shiga toxins (stx2)	Forward	TTTGTYACTGTSACAGCWGAAGCYTTACG	128
		Reverse	CCCCAGTTCARWGTRAGRTCMACRTC
		Probe-VIC	TCGTCAGGCACTGTCTGAAACTGCTCC
Listeria monocytogenes	hlyA-177	hlyA-177-F	TGCAAGTCCTAAGACGCCA	120
		hlyA-177-R	CACTGCATCTCCGTGGTATACTAA
	hlyA-146	hlyA-146-F	AAATCTGTCTCAGGYGATGT	113
		hlyA-146-R	CGATGATTTGAACTTCATCTTTTGC
Salmonella enteritidis	invA	invA-F	CACGCTCTTTCGTCTGGCA	154
		invA-R	TACGGTTCCTTTGACGGTGCGA
Staphylococcus aureus	FemB	FemB-F	AATTAACGAAATGGGCAGAAACA	93
		FemB-R	TGCGCAACACCCTGAACTT