Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq

Table 1

Primers and PCR settings used in this study.

Primer	Nucleotide sequence	Amplicon size (bp)	PCR condition
16s rRNA	F-TGGCTCAGATTGAACGCTGGCGGC	1500	Initial denaturation: 95 °C for 4 min, 35 cycles of denaturation: 95 °C for 40s, annealing: 64 °C for 40s, extension: 72 °C for 40s, and final extension: 72 °C for 5 min.
	R-TACCTTGTTACGACTTCACCCCA
blaOxa-51	F-TAATGCTTTGATCGGCCTTG	353	Denaturation: 94 °C for 5 min, 33 cycles of denaturation: 94 °C for 25s, annealing: 56.5 °C for 40s, extension: 72 °C for 50 s, and final extension: 72 °C for 6 min.
	R-TGGATTGCACTTCATCTTGG
blaSHV	F-CTT TAT CGG CCC TCA CTC AA	237	Initial denaturation: 94 °C for 4 min, 30 cycles of denaturation: 94 °C for 60s, annealing: 57.3 °C for 40s, extension: 72 °C for 70s, and final extension: 72 °C for 10 min.
	R-AGG TGC TCA TCA TGG GAA AG
blaTEM-1	F-CGC CGC ATA CAC TAT TCT CAG AAT GA	445	Initial denaturation: 94 °C for 4 min, 30 cycles of denaturation: 94 °C for 60s, annealing: 62.8 °C for 40s, extension: 72 °C for 70s, and final extension: 72 °C for 10 min
	R-ACG CTC ACC GGC TCC AGA TTT AT
blaCTX-M	F-ATG TGC AGY ACC AGT AAR GTK ATG GC	593	Initial denaturation: 94 °C for 4 min, 30 cycles of denaturation: 94 °C for 60s, annealing: 68.6 °C for 40s, extension: 72 °C for 70s, and final extension: 72 °C for 10 min
	R-TGG GTR AAR TAR GTS ACC AGA AYC AGC GG
blaAmpC	F-ACAGAGGAGCTAATCATGCG	1243	Initial denaturation: 94 °C for 4 min, 30 cycles of denaturation: 94 °C for 60s, annealing: 53.2 °C for 40s, extension: 72 °C for 60s, and final extension: 72 °C for 10 min
	R-GTTCTTTTAAACCATATACC
ISAba1-F	F-CACGAATGCAGAAGTTG	1507	Initial denaturation: 95 °C for 5 min, 35 cycles of denaturation: 95 °C for 45 s, annealing: 52 °C for 50s, extension: 72 °C for 50 s, and final extension: 72 °C for 10 min
AmpC-R	R-GTTCTTTTAAACCATATACC
-	TGAAGCTCCTGGGGATTCAC	-	Initial denaturation: 95 °C for 5 min, 30 cycles of: denaturation 95 °C for 60s, annealing: 40 °C for 60s, extension: 72 °C for 40s, and final extension: 72 °C for 5 min
	AAG TAA GTG ACT GGG GTG AGC G