Pasteurella multocida Toxin Aggravates Ligatured-Induced Periodontal Bone Loss and Inflammation via NOD-Like Receptor Protein 3 Inflammasome

Figure 1

NLRP3 expression is upregulated in periodontitis patients and mice. (a) Reconstructed 3D micro-CT images of the control and periodontitis groups. (b) BV, BV/TV, and BMD analysis of the control and periodontitis groups (/group). (c and f) H&E staining and immunohistochemistry staining of IL-1β and NLRP3 in the gingivae in control and periodontitis groups. Semiquantitative analysis (integrated optical density (IOD)/area) of IL-1β and NLRP3 immunohistochemistry staining of control and periodontitis groups. Values are presented as . (d) The distances between the CEJ and the ABC from the mesial side of the control and periodontitis groups (/group). (e) mRNA expression of IL-1β, IL-6, IL-10, and NLRP3 in the control and periodontitis groups by qRT-PCR (/group). GAPDH was used for normalization relative to control groups (, , and ). Abbreviations: ABC = alveolar bone crest; BMD = bone mineral density; BV = bone volume; BV/TV = bone volume/tissue volume ratio; CEJ = cemento-enamel junction; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; H&E = hematoxylin and eosin; IL = interleukin; micro-CT = microcomputed tomography; mRNA = messenger RNA; NLRP3 = NOD-like receptor family pyrin domain-containing 3; qRT-PCR = quantitative reverse transcription polymerase chain reaction; 3D = three-dimensional.

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