Pasteurella multocida Toxin Aggravates Ligatured-Induced Periodontal Bone Loss and Inflammation via NOD-Like Receptor Protein 3 Inflammasome

Figure 2

NLRP3 deficiency attenuates mice periodontitis in vivo. (a) Reconstructed 3D micro-CT images of periodontitis models of WT and Nlrp3^-/- mice. (b) The distances between the CEJ and the ABC from the mesial side of upper right second molars of WT and Nlrp3^-/- mice (/group). (c) BMD, BV, BV/TV, and resorbed bone volume analysis of WT and Nlrp3^-/- mice (/group). (d, f, and g) H&E staining and immunohistochemistry staining of IL-1β of WT and Nlrp3^-/- mice. The histogram shows the quantification of average optical density (integrated optical density (IOD)/area) of IL-1β immunohistochemistry staining of WT and Nlrp3^-/- mice groups (/group). (e) mRNA expression of IL-1β and IL-6 in the gingiva and alveolar bone of WT and Nlrp3^-/- mice (/group) by qRT-PCR. GAPDH was used for normalization relative to control groups. Values are presented as (, , and ). Abbreviations: ABC = alveolar bone crest; BMD = bone mineral density; BV = bone volume; BV/TV = bone volume/tissue volume ratio; CEJ = cemento-enamel junction; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; H&E = hematoxylin and eosin; IL = interleukin; micro-CT = microcomputed tomography; mRNA = messenger RNA; NLRP3 = NOD-like receptor family pyrin domain-containing 3; qRT-PCR = quantitative reverse transcription polymerase chain reaction; 3D = three-dimensional.

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