Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells

Figure 4

Shionone reduces inflammation and E. coli in SV-HUC-1 cells infected with urinary pathogenic E. coli. (a) The cell viability of each group. (b) The level of the inflammatory factor IL-1β in supernatant of cell in each group. (c) The level of the inflammatory factor IL-6 in the supernatant of cell in each group. (d) The level of the inflammatory factor TNF-α in the supernatant of cell in each group. (e) Staining of cytoskeleton, E. coli, and nuclei in SV-HUC-1 cells of each group of cell, with the cytoskeleton stained for phalloidin in red, E. coli in green, and nuclei stained for DAPI in blue. (f) Immunofluorescence intensity ratio of E. coli and DAPI. (g) Bacterial colony counts of each group. (h) Histogram statistics of bacterial CFU of each group. Compared with the control group, # means and ## means . Compared with the UPEC group, means and means .

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