Contrast Media & Molecular Imaging

Research Article

Labelling of Y- and Lu-DOTA-Bioconjugates for Targeted Radionuclide Therapy: A Comparison among Manual, Semiautomated, and Fully Automated Synthesis

Table 2

Evaluation of radiochemical purity of ⁹⁰Y- and ¹⁷⁷Lu-DOTATOC preparations performed by a manual, semiautomated (ADD-2), and automated (ML Eazy) approach.


	⁹⁰Y-DOTATOC	¹⁷⁷Lu-DOTATOC

Manual syntheses
Syntheses failed	0/77	0/43
Uncompliant preparations (RCP < 97%)	6/77	3/43
TLC
Free-radionuclide (%)	0.2	0.2
Hydrolysed products (%)	0.0	0.1
Labelled product (%)	99.8	99.7
HPLC
Free radionuclide + hydrolysed products	1.2	0.8
By-products	0.5	0.5
Labelled product	98.3	98.7

Semiautomated syntheses
Syntheses failed	1/63	0/88
Uncompliant preparations (RCP < 97%)	1/62	0/88
TLC
Free-radionuclide	0.23	0.25
Hydrolysed products	0.27	0.15
Labelled product	99.5	99.6
HPLC
Free radionuclide + hydrolysed products	21.3	1.0
By-products	0.4	0.2
Labelled product	98.3	98.8

Automated syntheses
Syntheses failed	1/24	1/26
Uncompliant preparations (RCP < 97%)	1/23	0/25
TLC
Free-radionuclide	0.2	0.3
Hydrolysed products	0.3	0.4
Labelled product	99.5	99.3
HPLC
Free radionuclide + Hydrolysed products	1.9	0.7
By-products	0.4	0.9
Labelled product	97.7	98.4