Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with ⁶⁴Cu Using NOTA and NODAGA

Table 2

⁶⁴Cu-NOTA-ZHER2:S1 and ⁶⁴Cu-NODAGA-ZHER2:S1 biodistribution in mice bearing HER2-positive (SKOV-3) xenografts.

	Uptake (% ID/g)
	2 h		6 h		24 h
	^64Cu-NOTA- ZHER2:S1	^64Cu-NODAGA- ZHER2:S1	^64Cu-NOTA- ZHER2:S1	^64Cu-NODAGA- ZHER2:S1	^64Cu-NOTA- ZHER2:S1	^64Cu-NODAGA- ZHER2:S1
Blood	1.0 ± 0.2	0.32 ± 0.05	1.4 ± 0.2	0.26 ± 0.04	1.15 ± 0.08	0.5 ± 0.1
Lung	3.0 ± 0.5	0.8 ± 0.1	5.9 ± 0.6	1.1 ± 0.2	4.7 ± 1.0	2.0 ± 0.6
Liver	10.9 ± 0.6	2.8 ± 0.5	17 ± 3	3.6 ± 0.5	11.3 ± 0.9	5 ± 1
Spleen	1.6 ± 0.2	0.56 ± 0.09	2.6 ± 0.7	0.63 ± 0.09	3.1 ± 0.5	1.2 ± 0.3
Stomach	3.3 ± 0.3	0.8 ± 0.1	5.2 ± 0.3	1.1 ± 0.2	3.5 ± 0.4	1.6 ± 0.4
Kidney	207 ± 35	290 ± 35	69 ± 7	226 ± 41	14 ± 1	110 ± 25
Tumor	17 ± 2	12 ± 3	17 ± 4	10 ± 2	10 ± 1	11 ± 4
Muscle	0.45 ± 0.06	0.16 ± 0.05	0.55 ± 0.09	0.14 ± 0.03	0.55 ± 0.04	0.25 ± 0.05
Bone	1.0 ± 0.1	0.35 ± 0.07	1.16 ± 0.05	0.30 ± 0.05	1.1 ± 0.2	0.5 ± 0.2

Data are presented as mean ± SD for four mice
Significant difference between ⁴Cu-NOTA-ZHER2:S1 and ⁶⁴Cu-NODAGA-ZHER2:S1 at the given time point.