Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with ⁶⁴Cu Using NOTA and NODAGA

Table 3

Comparison of ⁶⁴Cu-NOTA-ZHER2:S1 and ⁶⁴Cu-NODAGA-ZHER2:S1 tumor-to-organ ratios in nude mice bearing SKOV-3 xenografts. Data are presented as mean ± SD for four mice.

	Tumor-to-organ ratio
	2 h		6 h		24 h
	^64Cu-NOTA- ZHER2:S1	^64Cu-NODAGA- ZHER2:S1	^64Cu-NOTA-ZHER2:342	^64Cu-NODAGA- ZHER2:S1	^64Cu-NOTA-ZHER2:342	^64Cu-NODAGA-ZHER2:342
Blood	17 ± 2	38 ± 5	12 ± 2	36 ± 4	8 ± 2	21 ± 8
Lung	5.6 ± 0.4	15 ± 2	2.8 ± 0.5	9 ± 2	2.1 ± 0.5	5 ± 1
Liver	1.6 ± 0.2	4.4 ± 0.8	1.0 ± 0.1	2.6 ± 0.4	0.8 ± 0.1	2.1 ± 0.8
Spleen	10.4 ± 0.7	22 ± 2	6.5 ± 0.6	15 ± 3	3.2 ± 0.6	9 ± 3
Stomach	5.1 ± 0.5	15 ± 2	3.2 ± 0.8	8 ± 1	2.8 ± 0.7	7 ± 3
Kidney	0.08 ± 0.01	0.04 ± 0.01	0.24 ± 0.04	0.042 ± 0.007	0.7 ± 0.1	0.10 ± 0.04
Muscle	38 ± 1	78 ± 10	30 ± 4	70 ± 14	17 ± 3	43 ± 13
Bone	18 ± 2	35 ± 3	14 ± 3	32 ± 9	9 ± 3	23 ± 7

The values for ⁶⁴Cu-NOTA-ZHER2:S1 and ⁶⁴Cu-NODAGA-ZHER2:S1 significantly differed for each tissue at all time points.