Correlation of Somatostatin Receptor-2 Expression with Gallium-68-DOTA-TATE Uptake in Neuroblastoma Xenograft Models

Figure 5

(a) Example images of ¹²³I-MIBG SPECT/CT in the SK-N-BE(2), CHLA-15, and BE(2)C xenograft models. The white arrows denote localized tumor on the shoulder. (b) Scatter plots of the tumor-to-muscle () ratios of ¹²³I-MIBG uptake in the SK-N-BE(2), CHLA-15, and BE(2)C xenograft models derived from SPECT/CT imaging. One-way analysis of variance (ANOVA) with Tukey’s test was used for statistical analysis. <0.05; . (c) Scatter plots of the tumor-to-muscle () ratio of ¹²³I-MIBG autoradiography in the SK-N-BE(2), CHLA-15, and BE(2)C xenograft models. One-way ANOVA with Tukey’s test was used for statistical analysis. ; <0.01. (d) Western Blot of NET with homogenized CHLA-15, SK-N-BE(2), and BE(2)C xenografts. Three tumors were randomly selected from three groups for Western blot analysis. (e) Quantitative analysis of NET protein expression in CHLA-15, SK-N-BE(2), and BE(2)C xenografts. NET protein expression in Western blot images was quantified densitometrically using ImageJ software (NIH, USA) and normalized with respect to the corresponding expression of β-actin. Comparison of NET expression between different groups was analyzed by the nonparametric Kruskal-Wallis analysis with Dunn’s multiple comparison tests. No significant difference of NET expression was observed between CHLA-15, SK-N-BE(2), and BE(2)C xenografts.

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