Breast Tumor Microcalcification Induced by Bone Morphogenetic Protein-2: A New Murine Model for Human Breast Tumor Diagnosis

Figure 1

(a) Expression, purification, and bioactivity of rhBMP-2: the bacterial expression construct encoding amino acids 288–396 was used for expression of rhBMP-2 (modified from [14]). Following the renaturation and purification using heparin column, 0.5 μg of eluted protein samples (monomer and dimer) was analyzed by NuPAGE gel electrophoresis as shown (b). Lanes 1–4 with reducing agent and lanes 5–8 without reducing agent. C2C12 cells were treated with various doses of purified rhBMP-2. After 72 h in culture, the alkaline phosphatase activity was measured from cell lysates shown in (c) and directly by colorimetric assay (d).