Tumor-Shed Antigen Affects Antibody Tumor Targeting: Comparison of Two ⁸⁹Zr-Labeled Antibodies Directed against Shed or Nonshed Antigens

Figure 1

Procedure of a new normalized length analysis method for autoradiography. Step 1: draw lines on autoradiography image imported into ImageJ. Step 2: export each line plot data file and plot the original intensity versus line length profile. Step 3: normalize each length and replot with the interpolation function, interp1, in Matlab. Step 4: plot mean and standard deviation intensity profile with normalized length. Step 5: plot normalized intensity profile with normalized length. Step 6: repeat steps 1–5 for each tumor section and get mean normalized intensity profile to represent tumor sections cut at 25%, 50%, and 75% regions of the long tumor axis.