Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase

Figure 6

Identification of each cell population in organs postmortem. The liver, the spleen, and tumour-containing musculoskeletal (MS) tissue samples were harvested from mice and imaged with a protocol that allows the identification of FLuc (shown in step 2) or LSSmO_NLuc cells (shown in step 4). Here, livers contained foci of RAW cells expressing FLuc, the spleen contained no exogenously administered cells, and the tumour-containing musculoskeletal tissue consisted of MSCs expressing LSSmO_NLuc. The radiance scale is the same for all images.