Sensitivity of Multiphase Pseudocontinuous Arterial Spin Labelling (MP pCASL) Magnetic Resonance Imaging for Measuring Brain and Tumour Blood Flow in Mice

Figure 1

Optimisation of EPI readout using respiratory triggering to acquire the imaging readout during the plateau phase of respiration, reducing signal fluctuation at the base of the brain. Black trace = raw respiration trace; red trace = digitised respiration trace which acts as the trigger signal; and blue trace = readout of the post-trigger delay period, followed by the imaging slice acquisition. A variable encoding the post-trigger delay was implemented in the sequence to allow adjustment for changing respiration rates.