Preclinical Evaluation of Radioiodinated Hoechst 33258 for Early Prediction of Tumor Response to Treatment of Vascular-Disrupting Agents

Figure 3

Evaluation of specificity of ¹³¹I-H33258 binding to necrotic tissue. (a) Uptake of ¹³¹I-H33258 in nonblocked and 10 mg/kg H33258 blocked viable and necrotic muscle. Data are % ID/g, expressed as mean ± SD () at 3 h postinjection of ¹³¹I-H33258. (b) Representative autoradiography (Aut) (upper panels) and corresponding macroscopic (middle panels) and microscopic (lower panels) H&E staining images of 10 μm partially necrotic muscle slices excised from mouse models blocking with or without H33258. Non-B: nonblocked; H-B: H33258 blocked; N: necrotic muscle; V: viable muscle. . Scale bar: 100 μm.

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