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Contrast Media & Molecular Imaging
Volume 2018, Article ID 5272014, 7 pages
https://doi.org/10.1155/2018/5272014
Research Article

Early Detection of Aβ Deposition in the 5xFAD Mouse by Amyloid PET

1Division of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea
2Radiological & Medico-Oncological Sciences, University of Science and Technology, Daejeon, Republic of Korea
3Division of Basic Radiation Bioscience, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea
4Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea
5Research Institute of Labeling, FutureChem Co., Ltd., Seoul, Republic of Korea
6Division of Medical Radiation Equipment, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

Correspondence should be addressed to Ji-Ae Park; rk.er.smarik@krapj and Jae Yong Choi; rk.er.smarik@ynahms

Received 18 September 2017; Revised 12 January 2018; Accepted 29 January 2018; Published 28 February 2018

Academic Editor: Anne Roivainen

Copyright © 2018 Se Jong Oh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. 18F-FC119S is a positron emission tomography (PET) tracer for imaging β-amyloid (Aβ) plaques in Alzheimer’s disease (AD). The aim of this study is to evaluate the efficacy of 18F-FC119S in quantitating Aβ deposition in a mouse model of early amyloid deposition (5xFAD) by PET. Method. Dynamic 18F-FC119S PET images were obtained in 5xFAD () and wild-type (WT) mice (). The brain PET images were spatially normalized to the M. Mirrione T2-weighted mouse brain MR template, and the volumes of interest were then automatically drawn on the cortex, hippocampus, thalamus, and cerebellum. The specific binding of 18F-FC119S to Aβ was quantified as the distribution volume ratio using Logan graphical analysis with the cerebellum as a reference tissue. The Aβ levels in the brain were also confirmed by immunohistochemical analysis. Result. For the 5xFAD group, radioactivity levels in the cortex, the hippocampus, and the thalamus were higher than those for the WT group. In these regions, specific binding was approximately 1.2-fold higher in 5xFAD mice than in WT. Immunohistochemistry supported these findings; the 5xFAD showed severe Aβ deposition in the cortex and hippocampus in contrast to the WT group. Conclusion. These results demonstrated that 18F-FC119S PET can successfully distinguish Aβ depositions in 5xFAD mice from WT.